The “EIA-THYROID-THYROGLOBULIN” is a one-step immunoassay, based on principle of “sandwich” method. The assay system utilizes a high affinity and specificity monoclonal antibody (enzyme conjugated and immobilized) directed against a distinct antigenic determinant on the intact TG molecule. The test sample is allowed to react simultaneously with the two antibodies, resulting in the TG molecules being sandwiched between the solid phase and enzyme-linked antibodies. After incubation, the wells are washed with washing solution to remove unbound labeled antibodies. A solution of TMB-Substrate is added and incubated, resulting in the development of a blue color. The color development is stopped with the addition of Stoping Reagent, changing the color to yellow. The concentration of TG is directly proportional to the color intensity of the test sample. Absorbance is measured spectrophoto metrically at 450 nm.
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