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Free T3 Hormones Kit

Free T3 Hormones Kit
Free T3 Hormones Kit
Product Description
The Bi-EIA-Thyroid-T3 free is a one-stepimmunoa say to determine the presence of free triodothyronine (free T3) inhuman serum using competitive micro plate enzyme immuno assay. Plates are coated with anti-T3antibodies. Serum reference, patient specimen, or control is first addedto microplate well. Enzyme-T3 conjugate is added. T3 present in the sample competes with Enzyme-T3 conjugate for binding with anti-Т3 coated micro plate to form an antigen-antibody complex. Unbound conjugateis removed by washing. The enzyme activity in the antibody-bound fraction is inversely proportional to the native free triiodothyronine concentration. The enzyme activity is revealed by a color change in TMB-Sub strate solution.
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